Raman Spectroscopy

Author(s): Volker Deckert

Raman based microspectroscopy has been recognized as a powerful tool to study biological samples like e.g. cells, tissues etc because the method provides molecular information without external markers such as stains or radioactive labels. Raman spectroscopy is a non destructive technique and does in general, require only minimal or no sample preparation. However while the specificity of Raman spectroscopy is very high its sensitivity i.e. the conversion efficiency of the Raman effect is rather poor. Since only a small amount of the incident photons are inelastically scattered, the detection of molecules being present in very low concentrations is limited. To overcome the disadvantage of low signal intensities from most biomolecules, special Raman signal enhancing techniques can be applied.

Download this tutorial

You need to login before you can download this tutorial.

Round Robin Experiment

Raman spectroscopy has already proved its effectiveness in many cases for medical diagnostics such as for cancer, cardiovascular diseases and infections. However, there are no standards in the different working groups, e.g. for sample preparation, implementation of the Raman experiments, spectra pre-treatment, data evaluation, etc.In a round robin experiment, the required groundwork will take place in order to define standardised Raman measurement methods, which will be fundamental for establishing Raman spectroscopy for clinical diagnostic procedures.

Latest News

5th International Conference on Biophotonics

20 March 2017

Continuing the success of the previous meetings held in Sacramento, Ottawa, Jena, and Florence, the 5th conference will come to Perth, Western Australia, running over two full days, and back-to-back with the Science on the Swan medical research conference, which delegates are strongly encouraged to also attend, at discounted rates. [more]

Biophotonics Wiki

Visit our wiki to consult our latest technologies and techniques. [more]


Subscribe to our newsletter and get information about Photonics- 4Life:

Photonics4Life RSS Feed