Raman Spectroscopy

Author(s): Volker Deckert

Raman based microspectroscopy has been recognized as a powerful tool to study biological samples like e.g. cells, tissues etc because the method provides molecular information without external markers such as stains or radioactive labels. Raman spectroscopy is a non destructive technique and does in general, require only minimal or no sample preparation. However while the specificity of Raman spectroscopy is very high its sensitivity i.e. the conversion efficiency of the Raman effect is rather poor. Since only a small amount of the incident photons are inelastically scattered, the detection of molecules being present in very low concentrations is limited. To overcome the disadvantage of low signal intensities from most biomolecules, special Raman signal enhancing techniques can be applied.

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Round Robin Experiment

Raman spectroscopy has already proved its effectiveness in many cases for medical diagnostics such as for cancer, cardiovascular diseases and infections. However, there are no standards in the different working groups, e.g. for sample preparation, implementation of the Raman experiments, spectra pre-treatment, data evaluation, etc.In a round robin experiment, the required groundwork will take place in order to define standardised Raman measurement methods, which will be fundamental for establishing Raman spectroscopy for clinical diagnostic procedures.

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20 March 2017

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